Figure 2.

A: The gross appearance of tumor mass present in a BALB/C mouse 28 days after inoculation with PC3 prostate cancer cells. B: Sporadic PC3 cells infiltrate into nerve tissue. The arrows indicate nerve tissue. H&E, 100×. C: Sporadic PC3 cells infiltrated the nerve. Both PC3 cells and nerve tissue express NGF. The red arrows indicated nerve tissue. NGF immunohistochemical staining (SP), 100×. D: Sporadic PC3 cells infiltrate the nerve. Both PC3 cells and nerve tissue expressed CXCL12. Red arrow indicates nerve tissue secreting CXCL12. Blue arrow indicates tumor cells. CXCL12 immunohistochemical staining (SP), 100×. E: Sporadic PC3 cells infiltrate the nerve. PC3 cells expresse CXCR4. The arrow indicate nerve tissue. CXCR4 immunohistochemical staining (SP), 100×. F: PC3 cells express MMP-2 that degrades ECM around nerve tissue and promotes PNI. The red arrow indicates nerve tissue. MMP-2 immunohistochemical staining (SP), 200×. G: PC3 cells express MMP-9 that degrades ECM around nerve tissue and promotes PNI. The red arrow indicates the nerve tissue. MMP-9 immunohistochemical staining (SP), 200×.