Figure 1.

Effects of cilengitide on endothelial cell proliferation and apoptosis. (A) Adhesion and morphology. HMEC-1 endothelial cells grown on uncoated dishes were treated for 24 hours with control medium or cilengitide at concentrations of 1 μg/ml, 5 μg/ml and 50 μg/ml. Phase contrast micrographs were taken 24 hours after treatment. (B) Proliferation assay. Cells were treated with medium containing 4% FCS and cilengitide at the concentrations indicated, trypsinized and counted after 24, 48 and 72 hours incubation at 37°C. (C) Apoptosis. Floating and attached cells were collected 24 hours after treatment with cilengitide at the indicated concentrations. Apoptosis was assessed by flow cytometry after annexin V and propidium iodide staining. Experiments were performed in triplicate.