Research

Down-regulated miR-9 and miR-433 in human gastric carcinoma

Hongchun Luo¹ , Hongbin Zhang² , Zhenzhen Zhang³ , Xia Zhang¹ , Bo Ning¹ , Jinjun Guo¹ , Na Nie⁴ , Bo Liu⁴ and Xiaoling Wu¹

¹  Department of Gastroenterology, The Second Affiliated Hospital, Chongqing Medical University, Chongqing 400010, PR China

²  Department of Hematology, The First Affiliated Hospital, Chongqing Medical University, Chongqing 400016, PR China

³  The Key Laboratory of Molecular Biology of Infectious Diseases of Ministry of Education, Viral Hepatitis research Institute, Chongqing Medical University, Chongqing 400016, PR China

⁴  Department of Laboratory Medicine, Chongqing Medical University, Chongqing 400016, PR China

author email corresponding author email

Journal of Experimental & Clinical Cancer Research 2009, 28:82doi:10.1186/1756-9966-28-82

Published:	16 June 2009

Abstract

Background

MircoRNAs(miRNAs) are short, endogenously non-coding RNAs. The abnormal expression of miRNAs may be valuable for the diagnosis and treatment of tumors.

Methods

To screening the special miRNAs in gastric carcinoma, expression level of miRNAs in gastric carcinoma and normal gaster samples were detected by miRNA gene chip. Then, the expressions of miR-9 and miR-433 in gastric carcinoma tissue and SGC7901 cell line were validated by qRT-PCR. GRB2 and RAB34, targets of miR-433 and miR-9 respectively, were detected by Western blot.

Results

We found 19 miRNAs and 7 miRNAs were down-regulated and up-regulated respectively. Compared with normal gaster samples, our data showed that miR-9 and miR-433 were down-regulated in gastric carcinoma. Meanwhile, we also found that miR-433 and miR-9 regulated the expression levels of GRB2 and RAB34 respectively.

Conclusion

Our data show miR-9 and miR-433 was down-regulated in gastric carcinoma. The targets of miR-433 and miR-9 were tumor-associated proteins GRB2 and RAB34 respectively. This result provided the related information of miRNAs in gastric carcinoma.