Representative cytotoxicity experiments against the low Trop-2 expressing cell line. UMMT-ARK-1 (2 a) and the high Trop-2 expressing cell line OMMT-ARK-2 (2 b) at different effector to target cell ratios in the presence or absence of hRS7 in a 5 h 51Cr-release cytotoxicity assay. Consistent with their Trop-2 expression levels, low degrees of ADCC was detected against UMMT-ARK-1 while a high degree of ADCC was detected against the OMMT-ARK-2 cell line. Negligible cytotoxicity was detectable in the absence of hRS7 or in the presence of rituximab control antibody against both cell lines.
Raji et al. Journal of Experimental & Clinical Cancer Research 2011 30:106 doi:10.1186/1756-9966-30-106